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04 October 2022 | Story Leonie Bolleurs | Photo Leonie Bolleurs
Matseliso Monnapula, Dr Jana Vermaas, and Liezl van der Walt
Matseliso Monnapula, Dr Jana Vermaas, and Liezl van der Walt. They are all involved in a research project to grow a new textile that resembles leather.

Pure curiosity. 

That was what gave rise to the development of a new textile, which was created in the Textile Lab and later evaluated for consumer use in the Sensory Lab of the University of the Free State (UFS).

Matseliso Monnapula, a master’s student in the Division of Consumer Science, is experimenting with bacterial cellulose, which is produced as a by-product in the fermentation process when making kombucha. Her goal is to determine its efficacy as a possible sustainable textile alternative for use in the apparel industry.

She says finding this textile alternative was initially the result of pure curiosity. “My brother brews kombucha, so we always wondered in what other ways this fascinating mass of cellulose could be used.”

“It was upon further research that we discovered that there actually is more to it – from within the textile industry, biomedical and tissue engineering disciplines, paper and audio speaker manufacturing, to even the food industry,” states Monnapula.

She had a greater inclination towards its use in the textile industry and presented the idea to her supervisor, Dr Jana Vermaas, Lecturer in the Department of Sustainable Food Systems and Development. “From there it was all systems go,” remarks Monnapula. 

The interesting process of growing this textile entails brewing tea (black, green, or rooibos tea can be used for this purpose) and adding sugar, vinegar, or previously brewed kombucha to maintain a favourable pH level. “One then inoculates the sweetened tea with a starter culture of acetic acid bacteria and yeasts, also known as SCOBY (symbiotic culture of bacteria and yeasts). It is then left for two to four weeks under specific conditions, during which the fermentation process takes place. In this period, the cellulose gradually starts to form on the liquid’s surface,” explains Monnapula, who was assisted by her co-supervisor, Prof Celia Hugo from the Department of Microbiology and Biochemistry. 

Vegan leather

The process of making bacterial cellulose accounts for the many benefits of this leather-like textile. “The process and its aftermath are significantly less detrimental to the environment than most commercial textiles produced today. It is known that the textile industry is characterised by the excessive usage of chemicals, water, energy, and the generation of toxic effluent that is not always disposed of correctly, thereby affecting human, vegetal and animal well-being. Moreover, it eliminates animal cruelty, and in relation to real leather, it will also be more available and less expensive.”

“Secondly,” she states, “bacterial cellulose is biodegradable, which is one way of contributing towards a circular economy in the textile industry, while moving away from the traditional linear economy we know today.”

Within the apparel industry, this textile, which is mostly suitable for accessories, can be used to make products that are typically made of leather. For instance, bags, jackets, shoes, and bucket hats. 

From kombucha to leather-like textile
Samples of the new textile made from Kambucha. Photo: Leonie Bolleurs 

 

Versatile use

She states that according to their knowledge, the bacterial cellulose has not yet been grown in South Africa or Africa. However, it has been extensively researched in America and Europe. “There have been several experiments to make biodegradable packaging, facial masks in the cosmetics industry, sausage casings, and fruit rolls – and interestingly enough – it can even be enjoyed as a native Philippine dessert known as nata de coco. This goes to show how versatile it is,” she says.

Monnapula says there is still plenty of room for improvement and further development before reaching a point where she can introduce her work as a contender in the South African market. For instance, the waterproof capability of the textile is yet to be perfected. “More research is also necessary to enhance its hydrophobic and decreasing its hydrophilic properties.”

She is also of the opinion that further dyeing, using environmentally friendly methods and natural dyes to obtain a wider variety of colours, is necessary. 

Penetrating the market

Once it is ready, this textile will be a marketable product that can be manufactured for commercial use. “A few European start-up companies have recently managed to penetrate the market and introduce apparel made from bacterial cellulose. I believe that upon further development and modifications, we can eventually follow suit,” says Monnapula.

The bacterial cellulose textile was evaluated in the UFS Sensory Lab, a facility mostly used to test food products. Liezl van der Walt, Sensory Lab Manager, states that the Sensory Lab plays a crucial role in determining the consumer acceptance of new products as well as how the product can be improved. She believes that the textile project was just the beginning of many more textile-related sensory panels to take place. 


Within the apparel industry, this textile can be used to make products that are typically made of leather, including bags, jackets, shoes, and bucket hats. – Matseliso Monnapula

 


News Archive

UFS study on cell development in top international science journal
2008-09-16

A study from the University of the Free State (UFS) on how the change in the packaging of DNA with cell development influenced the expression of genes, will be published in this week’s early edition of the prestigious international, peer-reviewed science journal, the Proceeding of the National Academy of Sciences of the USA (PNAS).

The PNAS journal has an impact factor of 10, which means that studies published in the journal are, on average, referred to by ten other scientific studies in a two year period. The South African Journal of Science, by comparison, has an impact factor of 0.7.

The UFS study, funded by the Wellcome Trust and the National Research Foundation (NRF), looked at how the change in the packaging of DNA with cell development influenced the expression of genes. It is very relevant to research on stem cells, an area of medicine that studies the possible use of undifferentiated cells to replace damaged tissue.

Prof. Hugh Patterton, of the Department of Microbial, Biochemical and Food Biotechnology at the UFS, who led the study, said: "We are extremely proud of this study. It was conceived in South Africa, it was performed in South Africa, the data were analysed in South Africa, and it was published from South Africa."

When a gene is expressed, the information encoded in the gene is used to manufacture a specific protein. In eukaryotes, which include humans, there is approximately 1m of DNA, containing the genes, in every cell. This length of DNA has to fit into a cell nucleus with a diameter of only about 10 micrometer. In order to fit the DNA into such a small volume, eukaryotic cells wrap their DNA onto successive protein balls, termed nucleosomes. Strings of nucleosomes, resembling a bead of pearls, is folded into a helix to form a chromatin fiber. The study from the UFS investigated how the binding of a specific protein, termed a linker histone, that binds to the length of DNA between nucleosomes, influenced the formation of the chromatin fiber and also the activity of genes.

"We found that the linker histone bound to chromatin in yeast, which we use as a model eukaryote, under conditions where virtually all the genes in the organism were inactive. It was widely believed that the binding of the linker histone caused the inactivation of genes. We studied the relationship between the amount of linker histone bound in the vicinity of each gene and the expression of that gene for all the genes in yeast, using genomic techniques. We made the surprising discovery that even through the linker histone preferentially bound to genes under conditions where the genes were shut off, this inactivation of genes was not caused by the binding of the linker histone and folding of the chromatin,” said Prof. Patterton.

He said: “Instead our data strongly suggested that the observed anti-correlation was due to the movement of enzymes along the DNA molecule, involved in processing the information in genes for the eventual manufacture of proteins. This movement of enzymes displaced the linker histones from the DNA. This finding now requires a rethink on aspects of how packaging of DNA influences gene activity."

Prof. Patterton said that his research group, using the Facility for Genomics and Proteomics as well as the Bioinformatics Node at the UFS, was currently busy with follow-up studies to understand how other proteins in nucleosomes affected the activities of genes, as well as with projects to understand how chemicals found in red wine and in green tea extended lifespan. "We are certainly having a marvelous time trying to understand the fundamental mechanisms of life, and the UFS is an exciting place to be if one was interested in studying life at the level of molecules," he said.


Media Release
Issued by: Lacea Loader
Assistant Director: Media Liaison
Tel: 051 401 2584
Cell: 083 645 2454
E-mail: loaderl.stg@ufs.ac.za  
18 September 2008
 

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