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04 October 2022 | Story Leonie Bolleurs | Photo Leonie Bolleurs
Matseliso Monnapula, Dr Jana Vermaas, and Liezl van der Walt
Matseliso Monnapula, Dr Jana Vermaas, and Liezl van der Walt. They are all involved in a research project to grow a new textile that resembles leather.

Pure curiosity. 

That was what gave rise to the development of a new textile, which was created in the Textile Lab and later evaluated for consumer use in the Sensory Lab of the University of the Free State (UFS).

Matseliso Monnapula, a master’s student in the Division of Consumer Science, is experimenting with bacterial cellulose, which is produced as a by-product in the fermentation process when making kombucha. Her goal is to determine its efficacy as a possible sustainable textile alternative for use in the apparel industry.

She says finding this textile alternative was initially the result of pure curiosity. “My brother brews kombucha, so we always wondered in what other ways this fascinating mass of cellulose could be used.”

“It was upon further research that we discovered that there actually is more to it – from within the textile industry, biomedical and tissue engineering disciplines, paper and audio speaker manufacturing, to even the food industry,” states Monnapula.

She had a greater inclination towards its use in the textile industry and presented the idea to her supervisor, Dr Jana Vermaas, Lecturer in the Department of Sustainable Food Systems and Development. “From there it was all systems go,” remarks Monnapula. 

The interesting process of growing this textile entails brewing tea (black, green, or rooibos tea can be used for this purpose) and adding sugar, vinegar, or previously brewed kombucha to maintain a favourable pH level. “One then inoculates the sweetened tea with a starter culture of acetic acid bacteria and yeasts, also known as SCOBY (symbiotic culture of bacteria and yeasts). It is then left for two to four weeks under specific conditions, during which the fermentation process takes place. In this period, the cellulose gradually starts to form on the liquid’s surface,” explains Monnapula, who was assisted by her co-supervisor, Prof Celia Hugo from the Department of Microbiology and Biochemistry. 

Vegan leather

The process of making bacterial cellulose accounts for the many benefits of this leather-like textile. “The process and its aftermath are significantly less detrimental to the environment than most commercial textiles produced today. It is known that the textile industry is characterised by the excessive usage of chemicals, water, energy, and the generation of toxic effluent that is not always disposed of correctly, thereby affecting human, vegetal and animal well-being. Moreover, it eliminates animal cruelty, and in relation to real leather, it will also be more available and less expensive.”

“Secondly,” she states, “bacterial cellulose is biodegradable, which is one way of contributing towards a circular economy in the textile industry, while moving away from the traditional linear economy we know today.”

Within the apparel industry, this textile, which is mostly suitable for accessories, can be used to make products that are typically made of leather. For instance, bags, jackets, shoes, and bucket hats. 

From kombucha to leather-like textile
Samples of the new textile made from Kambucha. Photo: Leonie Bolleurs 

 

Versatile use

She states that according to their knowledge, the bacterial cellulose has not yet been grown in South Africa or Africa. However, it has been extensively researched in America and Europe. “There have been several experiments to make biodegradable packaging, facial masks in the cosmetics industry, sausage casings, and fruit rolls – and interestingly enough – it can even be enjoyed as a native Philippine dessert known as nata de coco. This goes to show how versatile it is,” she says.

Monnapula says there is still plenty of room for improvement and further development before reaching a point where she can introduce her work as a contender in the South African market. For instance, the waterproof capability of the textile is yet to be perfected. “More research is also necessary to enhance its hydrophobic and decreasing its hydrophilic properties.”

She is also of the opinion that further dyeing, using environmentally friendly methods and natural dyes to obtain a wider variety of colours, is necessary. 

Penetrating the market

Once it is ready, this textile will be a marketable product that can be manufactured for commercial use. “A few European start-up companies have recently managed to penetrate the market and introduce apparel made from bacterial cellulose. I believe that upon further development and modifications, we can eventually follow suit,” says Monnapula.

The bacterial cellulose textile was evaluated in the UFS Sensory Lab, a facility mostly used to test food products. Liezl van der Walt, Sensory Lab Manager, states that the Sensory Lab plays a crucial role in determining the consumer acceptance of new products as well as how the product can be improved. She believes that the textile project was just the beginning of many more textile-related sensory panels to take place. 


Within the apparel industry, this textile can be used to make products that are typically made of leather, including bags, jackets, shoes, and bucket hats. – Matseliso Monnapula

 


News Archive

Research by experts published in Nature
2011-06-02

 
The members of the research group are, from the left, front: Christelle van Rooyen, Mariana Erasmus, Prof. Esta van Heerden; back: Armand Bester and Prof. Derek Litthauer.
Photo: Gerhard Louw

A  research article on the work by a team of experts at our university, under the leadership of Prof. Esta van Heerden, and counterparts in Belgium and the USA has been published in the distinguished academic journal Nature today (Thursday, 2 June 2011).

The article – Nematoda from the terrestrial deep subsurface of South Africa – sheds more light on life in the form of a small worm living under extreme conditions in deep hot mines. It was discovered 1,3 km under the surface of the earth in the Beatrix Goldmine close to Welkom and is the first multi-cellular organism that was found so far beneath the surface of the earth. The worm (nematode) was found in between a rock face that is between 3 000 and 12 000 years old.

The research can shed some new light on the possibility of life on other planets, previously considered impossible under extreme conditions. It also expands the possibilities into new areas where new organisms may be found.

These small invertebrates live in terrestrial soil subjected to stress almost for 24 hours They live through sunshine, rain, scorching temperatures and freezing conditions. Through time they developed a means to cope with harsh conditions. Terrestrial nematodes (roundworms, not to be confused or related to earthworms) are among those very tough small invertebrates that deal with those conditions everywhere. After insects they are the most dominant multi-cellular (metazoan) species on the planet having a general size of 0,5 to 1 mm and are among the oldest metazoans on the planet, Nature says in a statement on the article.

They inhabit nearly every imaginable habitat form the deep seas to the acid in pitcher . Some nematodes simply eat bacteria and these are the ones we study here. Terrestrial nematodes have developed a survival stage that can take them through hard times (absence of food, extreme temperatures, too little oxygen, crowding, and more).

At the head of the research was Prof. Gaetan Borgonie of the Ghent University in Belgium and a world leader in the discipline of nematode research. He was brought into contact with the South African research leader, Prof. Esta van Heerden, who set up a cooperation agreement with the University of Ghent and Prof. Borgonie. Prof. Van Heerden manages the Extreme Biochemistry group at the UFS and the research was funded by several research grants.

The search for worms began in earnest in 2007, but it was soon clear that the sampling strategy was insufficient. A massive sampling campaign in 2008-2009 in several mines led to the discovery of several nematodes and the new nematode species Halicephalobus mephisto. It is named after the legend of Faust where the devil, also known as the lord of the underworld is called Mephistopheles.

Nature says special filters had to be designed and installed on various boreholes. Unfortunately, there is no easy way of finding a magic formula and designs had to be adapted by trial and error; improving existing designs all the time. The work of the UFS Mechanical Workshop, which manufactured, adapted and helped design it, was crucial in this respect. Filters were left on the holes for varying periods, sometimes for a few hours and sometimes for months. Prof. Derek Litthauer from the UFS played a big role in sampling, filter designs and coming up with ideas for names for the new nematode with Prof. Borgonie.

Research showed that the nematodes can live in the deep for up to 12 000 years. Three students – Armand Bester, Mariana Erasmus and Christelle van Rooyen from the UFS – did the work on this.

The importance of multi-cellular animals living in the ultra-deep subsurface is twofold: The nematodes graze on the existing bacterial population and influence their turnover. Secondly, if more complex multi-cellular organisms can survive in the deep subsurface on earth, this may be good news when looking for life on other planets where the surface is considered too inhospitable (e.g. Mars). Complex life forms can be found in ecosystems previously thought to be uninhabitable. Nature says this expands the possibilities into new areas where new organisms may be discovered.

Future research will focus on selective boreholes to look for more metazoans, so that a better idea of the complexity of the ecosystems there can be obtained. It will also look for metazoans in the deep subsurface on other continents to determine similarities and differences.

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