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12 October 2020 | Story Leonie Bolleurs | Photo Supplied
Adriaan van der Walt
Although several international studies have used temperature metrics to statistically classify their seasonal divisions, a study in which Adriaan van der Walt was involved, would be the first known publication in a South African context using temperature as classification metric.

Gone are the days when we as South Africans would experience a three-month spring season, easing into summer, and then cooling off for three months before we hit winter.

Adriaan van der Walt, Lecturer in the Department of Geography at the University of the Free State (UFS), focuses his research on biometeorology (a specialist discipline exploring the role and climate change in physical and human environments) as well as climatology and geographic information systems.

He recently published an article: ‘Statistical classification of South African seasonal divisions on the basis of daily temperature data’ in the South African Journal of Science.

In this study, which Van der Walt undertook with Jennifer Fitchett, a colleague from the University of the Witwatersrand, data on daily maximum and minimum temperatures was collected from 35 meteorological stations of the South African Weather Service, covering the period between 1980 and 2015.

They went to great lengths to ensure that they had a complete set of data before presenting it to demonstrate seasonal brackets.

First for South Africa

Their statistical seasonal brackets indicate that South Africans now experience longer summers (from October to March), autumn in April and May, winter from June to August, and spring in September.

Although considerable work has been done using rainfall to determine seasonality in Southern Africa, Van der Walt believes that these methods did not work well as there are too many inconsistencies in this approach, as identified by Roffe et al. (2019, South African Geographical Journal). To make matters more complicated – as a semi-arid region, and with desert conditions along the west coast – some regions do not have enough rainfall to use as a classifier.

Temperature, on the other hand, worked well in this study. “Temperature, by contrast, is a continuous variable, and in Southern Africa has sufficient seasonal variation to allow for successful classification,” says Van der Walt.

He continues: “Although several international studies used temperature metrics to statistically classify their seasonal divisions, this study would be the first known publication in a South African context using temperature as classification metric.”

Van der Walt says what we understand as seasons largely relates to phenology – the appearance of blossoms in spring, the colouration and fall of leaves in autumn, and the migration of birds as a few examples. “These phenological shifts are more sensitive to temperature than other climatic variables.”

Seasonal brackets

According to Van der Walt, they believe that a clearly defined and communicated method should be used in defining seasons, rather than just assigning months to seasons.

“One of the most important arguments of our work is that one needs to critically consider breaks in seasons, rather than arbitrarily placing months into seasons, and so we welcome any alternate approaches,” he says.

A number of sectors apply the temperature-based division to their benefit. “For example, in the tourism sector it is becoming increasingly important to align advertising with the season most climatically suitable for tourism,” says Van der Walt.

Temperature-based division is also used to develop adaptive strategies to monitor seasonal changes in temperature under climate change. However, Van der Walt points out that each sector will have its own way of defining seasons. “Seasonal boundaries should nevertheless be clearly communicated with the logic behind them,” he says.

News Archive

Research eradicates bacteria from avocado facility
2017-01-17

 Description: Listeria monocytogenes Tags: Listeria monocytogenes

Listeria monocytogenes as seen under an electron
microscope. The photo was taken with a transmission
electron microscope at the microscopy unit of the UFS.
Bacteriophages (lollipop-like structures) can be seen
next to the bacterial cells.
Photo: Supplied

“The aim of my project was to identify and characterise the contamination problem in an avocado-processing facility and then to find a solution,” said Dr Amy Strydom, postdoctoral fellow in the Department of Microbial Biochemical and Food Biotechnology at the University of the Free State (UFS).

Her PhD, “Control of Listeria monocytogenes in an Avocado-processing Facility”, aimed to identify and characterise the contamination problem in a facility where avocados were processed into guacamole. Dr Strydom completed her MSc in food science in 2009 at Stellenbosch University and this was the catalyst for her starting her PhD in microbiology in 2012 at the UFS. The research was conducted over a period of four years and she graduated in 2016. The research project was funded by the National Research Foundation.

The opportunity to work closely with the food industry further motivated Dr Strydom to conduct her research. The research has made a significant contribution to a food producer (avocado facility) that will sell products that are not contaminated with any pathogens. The public will then buy food that is safe for human consumption.


What is Listeria monocytogenes?

Listeria monocytogenes is a food-borne pathogenic bacterium. When a food product is contaminated with L. monocytogenes, it will not be altered in ways that are obvious to the consumer, such as taste and smell. When ingested, however, it can cause a wide range of illnesses in people with impaired immune systems. “Risk groups include newborn babies, the elderly, and people suffering from diseases that weaken their immune systems,” Dr Strydom said. The processing adjustments based on her findings resulted in decreased numbers of Listeria in the facility.

The bacteria can also survive and grow at refrigeration temperatures, making them dangerous food pathogens, organisms which can cause illnesses [in humans]. Dr Strydom worked closely with the facility and developed an in-house monitoring system by means of which the facility could test their products and the processing environment. She also evaluated bacteriophages as a biological control agent in the processing facility. Bacteriophages are viruses that can only infect specific strains of bacteria. Despite bacteriophage products specifically intended for the use of controlling L. monocytogenes being commercially available in the food industry, Dr Strydom found that only 26% of the L. monocytogenes population in the facility was destroyed by the ListexP100TM product. “I concluded that the genetic diversity of the bacteria in the facility was too high and that the bacteriophages could not be used as a control measure. However, there is much we do not understand about bacteriophages, and with a few adjustments, we might be able to use them in the food industry.”

Microbiological and molecular characterisation of L. monocytogenes

The bacteria were isolated and purified using basic microbiological culturing. Characterisation was done based on specific genes present in the bacterial genome. “I amplified these genes with polymerase chain reaction (PCR), using various primers targeting these specific genes,” Dr Strydom said. Some amplification results were analysed with a subsequent restriction digestion where the genes were cut in specific areas with enzymes to create fragments. The lengths of these fragments can be used to differentiate between strains. “I also compared the whole genomes of some of the bacterial strains.” The bacteriophages were then isolated from waste water samples at the facility using the isolated bacterial strains. “However, I was not able to isolate a bacteriophage that could infect the bacteria in the facility.

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