• Ramírez, CS, Tolmie, C, Opperman, DJ, González, PJ, Rivas, MG, Brondino, CD, Ferroni, FM (2021) Copper nitrite reductase from Sinorhizobium meliloti 2011: Crystal structure and interaction with the physiological versus a nonmetabolically related cupredoxin-like mediator. Protein Science 30: 2310– 2323 doi.org/10.1002/pro.4195
  • Aschenbrenner, JC, Ebrecht, A, Tolmie, C, Smit, MS, Opperman, DJ (2021) Structure of the fungal hydroxylase, CYP505A30, and rational transfer of mutation data from CYP102A1 to alter regioselectivity. Catalysis Science and Technology 11: 7359-7367 doi.org/10.1039/D1CY01348C
  • Baek, M, DiMaio, F, Anishchenko, I, Dauparas, J, Ovchinnikov, S, Lee, GR, Wang, J, Cong, Q, Kinch, LN, Dustin Schaeffer, R, Millán, C, Park, H, Adams, C, Glassman, CR, DeGiovanni, A, Pereira, JH, Rodrigues, AV, Van Dijk, AA, Ebrecht, AC, Opperman, DJ, Sagmeister, T, Buhlheller, C, Pavkov-Keller, T, Rathinaswamy, MK, Dalwadi, U, Yip, CK, Burke, JE, Christopher Garcia, K, Grishin, NV, Adams, PD, Read, RJ, Baker, D (2021) Accurate prediction of protein structures and interactions using a three-track neural network. Science 373: 871–876 doi.org/10.1126/science.abj8754
  • Wilbers, D, Brehm, J, Lewis, RJ, van Marwijk, J, Davies, TE, Morgan, DJ, Opperman, DJ, Smit, MS, Alcalde, M, Kotsiopoulos, A, Harrison, STL, Hutchings, GJ, Freakley, SJ (2021) Controlling product selectivity with nanoparticle composition in tandem chemo-biocatalytic styrene oxidation. Green Chemistry 23:4170-4180 doi.org/10.1039/D0GC04320F
  • Hollmann, F, Opperman, DJ, Paul, CE (2021) Biocatalytic reduction reactions from a chemist's perspective. Angewandte Chemie - International Edition 11:5644–566 doi.org/10.1002/anie.202001876
  • Nett, N, Duewel, S, Schmermund, L, Opperman, DJ (2021) A robust and stereocomplementary panel of ene-reductase variants for gram-scale asymmetric hydrogenation. Molecular Catalysis 502:111404 doi.org/10.1016/j.mcat.2021.111404
  • Ebrecht, AC, Aschenbrenner, JC, Smit, MS, Opperman, DJ (2021) Biocatalytic synthesis of non-vicinal aliphatic diols. Organic and Biomolecular Chemistry 19(2):439–445 doi.org/10.1039/D0OB02086A
  • Maseme, MJ, Pennec, A, van Marwijk, J, Opperman, DJ, Smit, MS (2020) CYP505E3: A novel self-sufficient ω-7 in-chain hydroxylase. Angewandte Chemie - International Edition 59(26): 10359–10362 doi.org/10.1002/anie.202001055
  • Guarneri, A, Westphal, AH, Leertouwer, J, van Berkel, WJH, Paul, CE (2020) Flavoenzyme-mediated regioselective aromatic hydroxylation with coenzyme biomimetics. ChemCatChem 12(5):1368–1375 doi.org/10.1002/cctc.201902044
  • Tolmie, C, Do Aido-Machado, R, Ferroni, FM, Smit, MS, Opperman, DJ (2020) Natural variation in the ‘control loop’ of bvmoafl210 and its influence on regioselectivity and sulfoxidation. Catalysts 10(3):339 doi.org/10.3390/catal10030339
  • Ebrecht, AC, van der Bergh, N, Harrison, STL, Sewell, BT, Opperman, DJ (2019) Biochemical and structural insights into the cytochrome P450 reductase from Candida tropicalis. Scientific Reports 9(1):20088 doi.org/10.1038/s41598-019-56516-6
  • Freakley, SJ, Kochius, S, van Marwijk, J, Smit, MS, Hutchings, GJ (2019) A chemo-enzymatic oxidation cascade to activate C–H bonds with in situ generated H2O2. Nature Communications 10(1):4178 doi.org/10.1038/s41467-019-12120-w
  • Vorster, A, Smit, MS, Opperman, DJ (2019) One-pot conversion of cinnamaldehyde to 2-phenylethanol via a biosynthetic cascade reaction. Organic Letters 21(17):7024–7027 doi.org/10.1021/acs.orglett.9b02611
  • Engel, J, Mthethwa, KS, Opperman, DJ, Kara, S (2019) Characterization of new Baeyer-Villiger monooxygenases for lactonizations in redox-neutral cascades. Molecular Catalysis 468:44–51 doi.org/10.1016/j.mcat.2019.02.006
  • Josa-Culleré, L, Lahdenperä, ASK, Ribaucourt, A, Höfler, GT, Gargiulo, S, Liu, Y-Y., Xu, J-H, Cassidy, J, Paradisi, F, Opperman, DJ, Hollmann, F, Paul, CE (2019) Synthetic biomimetic coenzymes and alcohol dehydrogenases for asymmetric catalysis. Catalyst 9(3):207 doi.org/10.3390/catal9030207
  • Tolmie, C, Smit, MS, Opperman, DJ (2019) Native roles of Baeyer-Villiger monooxygenases in the microbial metabolism of natural compounds. Natural Product Reports 36(2):326–353 doi.org/10.1039/C8NP00054A
  • Dithugoe, CD, van Marwijk, J, Smit, MS, Opperman, DJ (2019) An alcohol dehydrogenase from the short-chain dehydrogenase/reductase family of enzymes for the lactonization of hexane-1,6-diol. ChemBioChem 20(1):96–102 doi.org/10.1002/cbic.201800533
  • Opperman, DJ, Murgida, DH, Dalosto, SD, Brondino, CD, Ferroni, FM (2019) A three-domain copper-nitrite reductase with a unique sensing loop. IUCrJ 6:248–258 doi.org/10.1107/S2052252519000241
  • Tolmie, C, Smit, MS and Opperman, DJ (2018) Alternative splicing of the aflatoxin-associated Baeyer–Villiger monooxygenase from Aspergillus flavus: Characterisation of MoxY isoforms. Toxins. 10:521 doi.org/10.3390/toxins10120521
  • Kochius, S, van Marwijk, J, Ebrecht, AC, Opperman, DJ, Smit, MS (2018) Deconstruction of the CYP153A6 alkane hydroxylase system: Limitations and optimization of in vitro alkane hydroxylation. Catalysts. 8:531 doi.org/10.3390/catal8110531
  • Hollmann, F, Kara, S, Opperman, DJ, Wang, Y (2018) Biocatalytic synthesis of lactones and lactams. Chemistry – An Asian Journal. 13:3601-3610 doi.org/10.1002/asia.201801180
  • Opperman DJ (2017) Structural investigation into the C-terminal extension of the ene-reductase from Ralstonia (Cupriavidus) metallidurans. Proteins. 85:2252-2257 doi.org/10.1002/prot.25372
  • Mthethwa, KS, Kassier, K, Engel, J, Kara, S, Smit, MS, Opperman DJ (2017) Fungal BVMOs as alternatives to cyclohexanone monooxygenase. Enzyme and Microbial Technology 106:11-17 doi.org/10.1016/j.enzmictec.2017.06.017
  • Ferroni, FM, Tolmie, C, Smit, MS, Opperman, DJ (2017) Alkyl formate ester synthesis by a fungal Baeyer-Villiger monooxygenase. ChemBioChem 18:515-517 doi.org/10.1002/cbic.201600684
  • Tosstorff, A, Kroner, C, Opperman, DJ, Hollmann, F, Holtmann, D (2017) Towards electro enzymatic processes involving old yellow enzymes and mediated cofactor regeneration.
    Engineering in Life Sciences 17:71-76 doi: 10.1002/elsc.201600158
  • Ferroni FM, Tolmie C, Smit MS, Opperman DJ (2016) Structural and catalytic characterization of a fungal Baeyer-Villiger monooxygenase. PloS one 11:e0160186 doi.org/10.1371/journal.pone.0160186
  • Pennec A, Jacobs CL, Opperman DJ, Smit MS (2015) Revisiting cytochrome P450 mediated oxyfunctionalization of linear and cyclic alkanes. Advanced Synthesis and Catalysis. 357:118-130 doi.org/10.1002/adsc.201400410
  • Pennec A, Hollmann F, Smit MS, Opperman DJ (2015) One-pot conversion of cycloalkanes to lactones. ChemCatChem. 7:236-239 doi.org/10.1002/cctc.201402835


BLOEMFONTEIN CAMPUS FACULTY CONTACT

Elfrieda van den Berg (Marketing Manager)
T: +27 51 401 2531
E:vdberge@ufs.ac.za

QWAQWA CAMPUS FACULTY CONTACT

Dilahlwane Mohono (Faculty Officer)
T: +27 58 718 5284
E:naturalscienceqq@ufs.ac.za

Home new

We use cookies to make interactions with our websites and services easy and meaningful, to better understand how they are used and to tailor advertising. You can read more and make your cookie choices here. By continuing to use this site you are giving us your consent to do this.

Accept